|
|||||
|
|
Genetically encoded photocrosslinkers as molecular probes to study G-protein-coupled receptors (GPCRs) |
|
| Authors: | Beck-Sickinger Annette G Budisa Nediljko |
| Affiliation: | 1. AK Biochemie und Biooranische Chemie, Institut für Biochemie, Universit?t Leipzig, Brüderstrasse 34, 04103 Leipzig (Germany) http://www.biochemie.uni‐leipzig.de/agbs;2. AK Biokatalyse, Insitut für Chemie, Technische Universit?t Berlin, Franklinstrasse 29, 10587 Berlin (Germany) http://www.biocat.tu‐berlin.de |
| Abstract: | The genetic code was expanded with orthogonal pairs to introduce photoactivatable amino acids into G-protein-coupled receptors (GPCRs) in a noninvasive manner. In this way the receptor surface could be mapped by searching for specific ligand interaction sites and the complex dynamics could be studied. This method is also useful for probing the structure of GPCR complexes in living cells. |
| Keywords: | amino acids photoactivation photochemistry receptors receptor–ligand interactions |
| 本文献已被 PubMed 等数据库收录！ | |