高速逆流色谱法分离纯化续随子种子中的七叶内酯

建立了高速逆流色谱(HSCCC)技术分离纯化续随子种子中七叶内酯的方法。将续随子种子的乙酸乙酯萃取物直接进行高速逆流色谱分离,考察了不同溶剂系统的分离效果。结果表明,最佳的溶剂系统为氯仿-甲醇-水(体积比为4:3:2),以其上相为固定相,下相为流动相。从200 mg续随子种子乙酸乙酯萃取物中分离得到80 mg七叶内酯,纯度为99.04%。HSCCC技术可高效分离纯化续随子种子中的七叶内酯,为得到高纯度的七叶内酯提供了制备技术。

Isolation and purification of esculetin from the seeds of Euphorbia lathyris L. using high-speed counter-current chromatography

A method for the isolation and purification of esculetin from the seeds of Euphorbia lathyris L. was developed using high-speed counter-current chromatography (HSCCC). The ethyl acetate extract of the seeds of Euphorbia lathyris L. was separated by the HSCCC directly. Different solvent systems were investigated, and the results showed that the best solvent system was the two-phase solvent system composed of chloroform-methanol-water (4:3:2, v/v/v). The lower phase was used as the mobile phase and the upper phase was used as the stationary phase. A total of 80 mg esculetin with the purity of 99.04% was successfully obtained from 200 mg crude extract of the seeds of Euphorbia lathyris L. The results indicate that optimized HSCCC offers a preferred method for the preparation of esculetin from the seeds of Euphorbia lathyris L.