A spectrophotometric assay for solid phase primary amino groups |
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Authors: | Violeta G Janolino Harold E Swaisgood |
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Institution: | 1. Southeast Dairy Foods Research Center, Department of Food Science, North Carolina State University, 27695-7624, Raleigh, NC
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Abstract: | A rapid, sensitive, and convenient spectrophotometric assay was developed for the measurement of amino groups on solid supports.
This method is based on the reaction of amino groups of solids with an excess ofo-phthaldialdehyde (OPA) and subsequent quantitative determination of unreacted OPA by reaction with glycine. Four solids possessing
variable quantities of amino groups were examined. Results indicate that about 70% of the total surface amino concentration
(determined by the microKjeldahl method) are available for ligand attachment. Unlike the spectrophotometric 2,4,6-trinitrobenzenesulfonic
acid method, the OPA spectrophotometric assay is more rapid, sensitive, and convenient, and unlike the spectrofluorimetric
OPA, it does not require sophisticated instrumentation. |
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Keywords: | Index Entries" target="_blank">Index Entries Solid phase amino groups assay of o-phthaldialdehyde assay spectrophotometric assay of solid phase amino groups |
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