| Synthesis and Expression of a Gene From Kringle-2 Domain of Tissue Plasminogen Activator in E. coli |
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| 作者姓名： | 华子春 傅和亮 陈于红 余瑞荣 王进 朱德煦 |
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| 作者单位： | Department of Biochemistry，Nanjing University，Nanjing 210008，PRC，Department of Biochemistry，Nanjing University，Nanjing 210008，PRC，Department of Biochemistry，Nanjing University，Nanjing 210008，PRC，Department of Biochemistry，Nanjing University，Nanjing 210008，PRC，Department of Biochemistry，Nanjing University，Nanjing 210008，PRC，Department of Biochemistry，Nanjing University，Nanjing 210008，PRC |
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| 基金项目： | Project supported by a grant from the Chinese National Program of High Technology Development. |
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| 摘 要： | The DNA fragment corresponding to the tissue plasminogen activator (tPA) sequence 174-262 (Kringle-2 domain) has been synthesized by using the solid phase phosphotriester method. The Kringle-2 domain of human tPA was expressed in Escherichia colt by secretion into the periplasmic space using the Lpp-Lac promoter and PIN-Ⅲ OmpA2 signal sequence. About two thirds of the expression product was secreted into the periplasmic space , and purified with ammonium sulfate fractionation, affinity chro-matography on Lysine-Sepharose, and FPLC-Mono Q exchange chromatography. The amino acid composition observed from the Kringle-2 purified from E. coli is identical with that expected for the 174-262 fragment of human tPA. Radio binding assay shows that the recombinant Kringle-2 domain possesses the activity of fibrin binding.
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Synthesis and Expression of a Gene From Kringle-2 Domain of Tissue Plasminogen Activator in E. coli |
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| HUA Zi-Chun FU He-Liang CHEN Yu-HongYU Rui-Rong WANG Jin and ZHU De-Xu.Synthesis and Expression of a Gene From Kringle-2 Domain of Tissue Plasminogen Activator in E. coli[J].Science in China(Chemistry),1994(6). |
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| Authors: | HUA Zi-Chun FU He-Liang CHEN Yu-HongYU Rui-Rong WANG Jin and ZHU De-Xu |
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| Abstract: | |
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| Keywords: | tissue plasminogen activator (tPA) Kringle domain |
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