SERS标记免疫检测研究进展

表面增强拉曼光谱(SERS)用于标记免疫检测是标记免疫学与SERS相结合的一门新型的研究技术。20世纪70年代，SERS现象的发现与证实给拉曼光谱技术的研究注入了新的活力。SERS因具有高灵敏度、较高选择性以及适合水溶液物质结构研究等特点，近年来已在生物医学研究领域中显示出独特的潜在应用前景。在标记免疫领域，SERS标记免疫研究更是得到了迅速的发展，成为了国内外的研究热点。文章从SERS标记免疫检测灵敏度的提高、非特异性吸附的降低、多组分检测等三方面叙述了SERS标记免疫检测的原理、特点、存在问题及最新发展。归纳了目前提高SERS标记免疫检测灵敏度的研究技术，阐述了研究中非特异性吸附带来的负面影响，简介了实验室的多组分研究工作。同时，对SERS标记免疫技术未来的研究方向与发展前景进行了展望。

Progress in Labeled Immunoassay Based on SERS

Labeled immunoassay utilizing surface-enhanced Raman spectroscopy (SERS) is a new-style research technology. The discovery and confirmation of surface-enhanced Raman spectroscopy have made Raman spectroscopy a powerful tool in many research fields. SERS has the advantages such as high sensibility and selectivity, also it is suitable for the study of liquid substance. In recent years, it has shown potential application future in biomedical field, and also developed fast in labeled immunoassay. The principle, specialty, problem and recent advances of labeled immunoassay based on SERS were reviewed in terms of detection limits, non-specific adsorption and multi-analyte immunoassay. The latest advancement in the improvement of detection limits was summarized, along with the introduction of some work in our lab, as well as expatiating on the effect of non-specific adsorption. Finally, the development trends and application perspectives were discussed.