| 芯片瞬间等速电泳-筛分电泳偶联分析精确计算DNA长度 |
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| 引用本文： | 刘大渔,梁广铁,莫建坤,周小棉. 芯片瞬间等速电泳-筛分电泳偶联分析精确计算DNA长度[J]. 分析化学, 2010, 38(1) |
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| 作者姓名： | 刘大渔 梁广铁 莫建坤 周小棉 |
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| 作者单位： | 广州医学院附属市一人民医院检验科临床化学技术研究室,广州,510180;广东省第二人民医院检验科,广州,510317 |
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| 基金项目： | “十一五”重大专项(No.2008ZX10004-004);;国家自然科学基金(No.30870753);;广东省科技计划项目(No.2008B060600036)资助 |
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| 摘 要： | 建立了基于相对迁移时间比例的方法,依据待侧DNA片段相对于上位及下位内标的迁移时间比例进行长度预测。实验结果显示,DNA片段的相对迁移时间比例在不同分析条件下具有良好的重现性。通过建立相对迁移时间比例相对于DNA片段长度的对应关系公式,实现了芯片瞬间等速电泳条件下DNA片段长度的准确预测。实际样品分析证实这种基于相对迁移时间比例的计算方法简单可靠,适合于芯片tITP-CGE分析中DNA长度的精确判定。
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| 关 键 词： | 芯片电泳 瞬间等速电泳 DNA长度计算 迁移时间 样品盐浓度 |
Precise Prediction of Deoxyribonucleic Acid sizes with Transient Isotachophoreis-Capillary Gel Electrophoresis Analysis on a Microchip |
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| LIU Da-Yu,LIANG Guang-Tie,MO Jian-Kun,ZHOU Xiao-Mian. Precise Prediction of Deoxyribonucleic Acid sizes with Transient Isotachophoreis-Capillary Gel Electrophoresis Analysis on a Microchip[J]. Chinese Journal of Analytical Chemistry, 2010, 38(1) |
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| Authors: | LIU Da-Yu LIANG Guang-Tie MO Jian-Kun ZHOU Xiao-Mian |
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| Affiliation: | Laboratory of Advanced Clinical Chemical Technology;Guangzhou First Municipal People's Hospital;Affiliated Hospital of Guangzhou Medical College;Guangzhou 510180;Department of Laboratory Medicine;Guangdong No.2 Provincial People's Hospital;Guangzhou 510317 |
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| Abstract: | The migration-time in transient isotachophoresis (tITP) separation is affected by sample salinity as the dependence of ITP time on sample-zone conductivity.The sample-to-sample variation of migration-time in microchip tITP-CGE analysis is an undesired factor for precise DNA sizing.In this work,a DNA sizing method that based on relative migration-time proportion (RMP) was developed to eliminate the effect of sample salinity on sizing precision.RMP was defined as the ratio of the migration-time difference bet... |
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| Keywords: | Microchip electrophoresis Transient isotachophoresis Deoxyribonucleic acid sizing Migration time Sample salinity |
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