| 双波长微板法测定细胞悬浮液中的环氧化物 |
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| 引用本文： | 李从发,崔宏杰,吉爱国,田秀云,曲音波.双波长微板法测定细胞悬浮液中的环氧化物[J].分析科学学报,2004,20(3):245-247. |
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| 作者姓名： | 李从发 崔宏杰 吉爱国 田秀云 曲音波 |
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| 作者单位： | 山东大学微生物技术国家重点实验室,济南,250100;华南热带农业大学工学院,海南,571737;山东大学药学院,济南,250012;山东大学微生物技术国家重点实验室,济南,250100 |
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| 基金项目： | 国家自然科学基金(No.30270047)，山东省自然科学基金(No.Y2000C21) |
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| 摘 要： | 用双波长微板法研究了缓冲溶液与细胞悬浮液中吸光度与环氧化物浓度的关系，通过分析比较发现，在两种体系中的回归曲线相互平行。据此，可用缓冲体系中所测结果绘制标准曲线，计算细胞悬浮液中环氧化物的含量。在建立了双波长微板法测定混浊体系中环氧化物的基础上，环氧化物水解酶活力可以方便快速的检测。
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| 关 键 词： | 双波长微板法 细胞悬浮液 环氧化物 环氧化物水解酶 苯基环氧乙烷 |
| 文章编号： | 1006-6144(2004)03-0245-03 |
| 修稿时间： | 2003年1月10日 |
Determination of Epoxides in Cell Suspensions by Double-Wavelength Spectrophotometry |
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| LI Cong-fa.Determination of Epoxides in Cell Suspensions by Double-Wavelength Spectrophotometry[J].Journal of Analytical Science,2004,20(3):245-247. |
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| Authors: | LI Cong-fa |
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| Institution: | LI Cong-fa~ |
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| Abstract: | Using double-wavelength spectrophotometry in microtiter plate, the correlation between the absorption and epoxide concentration was studied and compared in phosphate buffer and bacterial cell suspension. It was found that the two regressive curves for the two systems were parallel to each other. And it was inferred and proved that epoxide concentration in cell suspensions could be calculated using the regressive curve in the buffer system as standard curve. By using the developed double-wavelength spectrophotometric method, epoxide hydrolase activity was assayed quickly and conveniently. |
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| Keywords: | Double-wavelength spectrophotometry Cell suspensions Epoxide Epoxide hydrolase Styrene oxide |
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