Determination of Gatifloxacin in Semen by HPLC with Diode-Array and Fluorescence Detection |
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| Authors: | M. L. Salazar Cavazos L. Y. Colunga González G. Gallegos de Lerma N. Waksman de Torres |
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| Affiliation: | 1. Departamento de Química Analítica, U.A.N.L. Facultad de Medicina, Madero y Aguirre Peque?o Col. Mitras Centro C.P, 64460, Mty, N.L, México
2. Departamento de Patología, U.A.N.L. Facultad de Medicina, Madero y Aguirre Peque?o Col. Mitras Centro C.P, 64460, Mty, N.L, México
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| Abstract: | The objective of this work was to develop an analytical HPLC method, using DAD and fluorescence detection, for determination of gatifloxacin in semen. A reversed-phase column was used with 90:10 water-acetonitrile, containing 10 mM TBA and 25 mM citric acid, as mobile phase. Semen was deproteinized with acetonitrile. Recovery was 95 ± 10%. The limits of quantification by DAD and fluorescence were 2.3 and 0.03 μg.mL?1 respectively, with RSD of 3.4% for DAD and 2.8% for fluorescence. The method with fluorescence detection was used for quantification of gatifloxacin in the semen of patients under treatment. |
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