2-Deoxyglucose as a selective agent for derepressed mutants of Pichia stipitis |
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Authors: | Hassan K Sreenath Thomas W Jeffries |
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Institution: | (1) Forest Products Laboratory, Institute of Microbial Biochemical Technology, One Gifford Pinchot Drive, 53705 Madison, WI;(2) Department of Bacteriology, University of Wisconsin-Madison, Madison, WI |
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Abstract: | The glucose analog 2-deoxyglucose (2-DOG) has been used to obtain mutants depressed for pentose metabolism. Some researchers
have used 2-DOG alone whereas others have used it in the presence of a glucose-repressible carbon source. We examined both
methods and screened mutant strains for improved use of xylose in the presence of glucose. Pichia stipitis mutants selected for growth on d-xylose in the presence of 2-DOG used xylose from a 1∶1 glucose:xylose mixture more rapidly than did their parents. One of
these mutants, FPL-DX26, completely consumed xylose in the presence of glucose and produced 33g/L ethanol in 45h from 80 g/L
of this sugar mixture. Mutants selected for growth on 2-DOG alone did not show significant improvement. Selection for growth
on d-xylose in the presence of 2-DOG has been useful in developing parental strains for further genetic manipulation.
The Forest Products Laboratory is maintained in cooperation with the University of Wisconsin-Madison. This article was prepared
by U.S. Goverment employees on official time, and it is therefore in the public domain and not subject to copyright. |
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Keywords: | Pichia stipitis mutation selection 2-deoxyglucose (2-DOG) fermentation ethanol glucose repression |
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