HPLC of synthetic polymers characterization of polystyrenes by high performance precipitation liquid chromatography (HPPLC) |
| |
Authors: | R Schultz H Engelhardt |
| |
Institution: | 1. Angewandte Physikalische Chemie, Universit?t des Saarlandes, 6600, Saarbrücken, FRG
|
| |
Abstract: | Summary The advantages and disadvantages of high performance precipitation liquid chromatography have been demonstrated for polystyrene
homopolymers. Depending on the mobile phase composition at the dissolution point of the polymeric sample and surface properties
of the stationary phase, elution is governed either by a solution process or by adsorption. A contribution by adsorption was
noticed on silica as well as on reversed phases based on silica with a normal phase gradient of increasing polarity (heptane
to dichloromethane). Elution was solely governed by solubility of the polymers on both types of stationary phase for polystyrenes
with a molecular weight above 35 000 and reversed phase gradient of decreasing polarity (methanol to dichloromethane). Under
these conditions an identical dependence of elution solvent composition on sample size was found as for turbidity titrations.
Due to differences in the velocity of the eluent front and the polymeric sample with porous stationary phases the polymers
can be eluted as colloidal solutions Non-porous stationary phases are superior in this respect because the velocities of eluent
and solutes are identical. |
| |
Keywords: | High performance precipitation liquid chromagraphy Polystyrenes Non-porous stationary phases Elution conditions Colloidal elution |
本文献已被 SpringerLink 等数据库收录! |