微径高效液相色谱法分离三唑类手性化合物的对映体

 采用微径高效液相色谱技术在手性纤维素色谱柱上分离了一系列 1 1,2 ,4 三唑类手性化合物。比较了微径液相色谱与传统液相色谱的分离结果 ,研究了化合物的苯环上不同取代基及取代基位置对分离的影响 ,并探讨了分离机理。

Enantiomeric Separations of 1-1,2,4-Triazole Compounds by Micro-High Performance Liquid Chromatography

The enantiomeric separations of sixteen novel 1-1,2,4-triazole chiral compounds were investigated by micro-HPLC with a 200 mm x 100 microns i.d. capillary chiral OD column. The detection was performed at 223 nm. The mobile phase was a mixture of iso-propyl alcohol and n-hexane(20:80, V/V) at a flow rate of 30 microL/min and column head pressure of 6.9 MPa. Some of the enantiomers could be separated on baseline. In comparing with the separation results by the conventional HPLC method, the micro-HPLC method had the advantages of high-speed, economy, environmental friendliness etc. The effect of solute structure on enantio-selectivity was investigated and the chiral recognition mechanism was also discussed. It was found that the main separation mechanisms involve the pi-pi association and inclusion between the substituent R and the spiral pocket of OD-CSP.