| 极大螺旋藻中β-胡萝卜素的分离纯化及定量测定 |
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| 引用本文： | 胡朝辉,刘志礼.极大螺旋藻中β-胡萝卜素的分离纯化及定量测定[J].色谱,2001,19(1):85-87. |
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| 作者姓名： | 胡朝辉 刘志礼 |
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| 作者单位： | 南京大学生物科学与技术系藻菌工程实验室, |
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| 摘 要： | 极大螺旋藻 (Spirulinamaximum)中含有多种营养物质 ,其中 β 胡萝卜素具有重要的应用价值。利用 β 胡萝卜素极性小的特点 ,用丙酮 甲醇 (体积比为 7∶2 )溶液对其提取后皂化。采用石油醚萃取 ,通过氧化铝柱进行色谱分析 ,用反相高效液相色谱法 (RP HPLC法 )测定了 β 胡萝卜素的含量。方法准确性高、重现性好 ,平均回收率为96 8%。
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| 关 键 词： | 反相高效液相色谱法 极大螺旋藻 β胡萝卜素 中性氧化铝柱 |
| 文章编号： | 1000-8713(2001)01-0085-03 |
| 修稿时间： | 2000年4月1日 |
Determination and
Purification of |
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| HU Zhao hui,\ LIU Zhi li.Determination and
Purification of[J].Chinese Journal of Chromatography,2001,19(1):85-87. |
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| Authors: | HU Zhao hui \ LIU Zhi li |
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| Institution: | Lab of Algal and Bacterial Technology, Department of Biological Science and Technology, Nanjing University, Nanjing 210093, China. hzhbio@263.net |
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| Abstract: | There are several kinds of nutritious materials in Spirulina maximum, and beta-carotene is one of the most important materials. Because beta-carotene is of low polarity, for purification it was extracted with the mixture of acetone and methanol in the ratio of 7:2 (V/V), then saponified, and extracted with petroleum ether, purified with column packed with neutral Al2O3. Its content was determined by reversed-phase HPLC. This method is accurate and the results are reproducible. The average recovery was 96.8%. |
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| Keywords: | NULL |
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