Sampling considerations in supercritical fluid chromatography |
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Authors: | R. J. Skelton Jr. C. C. Johnson L. T. Taylor |
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Affiliation: | (1) Department of Chemistry, Virginia Polytechnic Institute and State University, 24061 Blacksburg, VA, USA |
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Abstract: | Summary Packed column supercritical fluid chromatography, like HPLC, utilizes a sample loop to introduce materials onto the column for analysis. Unlike HPLC the mobile phase in SFC cannot be used to dissolve the sample. In practice, this causes a solvent peak, which can create a problem in the chromatographic interpretation. This paper describes one approach to solving this problem. A valving scheme is used to extract materials with the supercritical CO2 mobile phase and introduce them onto the column with no external handling. The viability of this method is demonstrated and separations of the CO2 extracts for several materials are shown on various columns. Comparisons are made for coal and coffee extracts using this on-line method and conventional off-line CH2Cl2 extracts. Advantages of the on-line procedure as they apply to chromatography and high information detectors are also discussed. |
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Keywords: | Supercritical fluid chromatography Packed columns On-line sampling Supercritical CO2 extraction |
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