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Surfactant protein C metabolism in human infants and adult patients by stable isotope tracer and mass spectrometry |
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| Authors: | Manuela Simonato Aldo Baritussio Barbara Pioselli Carlo Ori Silvia Catinella Virgilio P. Carnielli Paola E. Cogo |
| Affiliation: | 1. Women’s and Children’s Health Department, University of Padova, 35128, Padova, Italy 2. Department of Medicine, University of Padova, 35128, Padova, Italy 3. Corporate Preclinical R&D, Analytics and Early Formulations Department, Chiesi Farmaceutici, 43122, Parma, Italy 4. Department of Medicine, Anesthesiology and Intensive Care, University of Padova, 35128, Padova, Italy 5. Division of Neonatology, Department of Mother and Child Health, Ospedale Salesi-Azienda Ospedaliero Universitaria Ospedali Riuniti, 60123, Ancona, Italy 6. Department of Medical Sciences, Polytechnic University of Marche, 60121, Ancona, Italy 7. Pediatric Cardiac Anesthesia/Intensive Care Unit, Department of Pediatric Cardiology and Cardiac Surgery, Bambino Gesù Children’s Hospital, 00165, Rome, Italy |
| Abstract: | Surfactant protein C (SP-C) is deemed as the surfactant protein most specifically expressed in type II alveolar epithelial cells and plays an important role in surfactant function. SP-C turnover in humans and its meaning in the clinical context have never been approached. In this study, we used mass spectrometry to investigate SP-C turnover in humans. We studied four infants and eight adults requiring mechanical ventilation. All patients had no lung disease. Patients received a 24-h continuous infusion of 13C-leucine as precursor of SP-C, and serial tracheal aspirates and plasma samples were obtained every 6 h till 48 h. SP-C was isolated from tracheal aspirates by sorbent-phase chromatography. 13C-leucine SP-C enrichment could be successfully measured in three infant and in four adult samples by using mass spectrometry coupled with a gas chromatographer. Median SP-C fractional synthesis rate, secretion time, and peak time were 15.7 (14.1–27.5) %/day, 6.0 (4.7–11.5) h, and 24 (20–27) h. In conclusion, this study shows that it is feasible to accurately determine SP-C turnover in humans by stable isotopes. |
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