| 重组鼠疫汉坦钩端多抗原位点同源合成基因的表达及活性鉴定 |
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| 引用本文： | 浦昀,齐燕飞,徐国良,徐卉,张士尧,盂日增,宋秀玲,杨怀宁,李娟. 重组鼠疫汉坦钩端多抗原位点同源合成基因的表达及活性鉴定[J]. 高等学校化学学报, 2011, 32(1): 84-87 |
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| 作者姓名： | 浦昀 齐燕飞 徐国良 徐卉 张士尧 盂日增 宋秀玲 杨怀宁 李娟 |
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| 作者单位： | 吉林大学公共卫生学院;吉林省出入境检验检疫局;吉林大学第一医院心血管中心; |
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| 基金项目： | 国家“重大新药创制”科技重大专项项目(批准号:2009ZX09103-105); 高等学校博士学科点专项科研基金(批准号:20090061120093); 国家质量监督检验检疫总局科技计划项目(批准号:2009IK214); 吉林大学研究生创新研究计划项目(批准号:20091028)资助 |
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| 摘 要： | 将人工合成的含鼠疫耶尔森氏菌的2个抗原位点、汉坦病毒的5个抗原位点和钩端螺旋体的5个抗原位点的串联基因片段克隆到原核表达载体pET-20b中,构建重组表达质粒pET-rYHL.转化E.coli BL21(DE3)后获得表达菌株.表达菌株经IPTG诱导后,可高效表达带组氨酸标签的以包涵体形式存在的融合蛋白.包涵体蛋白经尿...
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| 关 键 词： | 鼠疫 汉坦病毒 钩端螺旋体 合成基因 基因表达 活性鉴定 |
| 收稿时间： | 2010-06-13 |
Expression and Preliminary Activity Identification of Several Linked Antigen Epitopes of Consensce Genes of Yersinia Pestis,Hantavirus and Leptospira |
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| PU Yun,Qi Yan-Fei,XU Guo-Liang,XU Hui,ZHANG Shi-Yao,MENG Ri-Zeng,SONG Xiu-Ling,YANG Huai-Ning,LI Juan. Expression and Preliminary Activity Identification of Several Linked Antigen Epitopes of Consensce Genes of Yersinia Pestis,Hantavirus and Leptospira[J]. Chemical Research In Chinese Universities, 2011, 32(1): 84-87 |
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| Authors: | PU Yun Qi Yan-Fei XU Guo-Liang XU Hui ZHANG Shi-Yao MENG Ri-Zeng SONG Xiu-Ling YANG Huai-Ning LI Juan |
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| Affiliation: | PU Yun1,2,QI Yan-Fei1,XU Guo-Liang3,XU Hui3,ZHANG Shi-Yao1,MENG Ri-Zeng1,SONG Xiu-Ling1,YANG Huai-Ning2,LI Juan1(1.School of Public Health,Jilin University,Changchun 130021,China,2.Jilin Entry & Exit Inspection and Quarantine Bureau,Changchun 130062,3.Cardiovascular Center,First Hospital of Jilin University,China) |
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| Abstract: | The expression vector pET-rYHL was constructed by inserting the linked gene contained two antigen epitopes of F1 capsule antigen from yersinia pestis,five antigen epitopes of hantavirus and five antigen epitopes of leptospira into pET-20b,and was identified by digestion with restriction enzymes and sequence analysis.Then an expression strain was selected after transformation of the recombined plasmid into E.coli BL21(DE3),fusion protein with His-tag was efficiently expressed in the form of inclusion body af... |
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| Keywords: | Plague Hantavirus Leptospira Chimeric gene Gene expression Activity identification |
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