β-Xylosidase recovery by reversed micelles |
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Authors: | Hasmann Francislene Andrea Pessoa Adalberto Roberto Ines Conceicao |
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Institution: | 1.Department of Biotechnology, FAENOUIL, Rod. Itajubá-Lorena, Km 74.5, CEP 12.600-000, Lorena, SP, Brazil ;2.Department of Biochemical and Pharmaceutical Technology/FCF, University of S?o Paulo, PO Box 66083, CEP 05315-970, S?o Paulo, SP, Brazil ; |
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Abstract: | β-Xylosidase, an enzyme produced by Penicillium janthinellum fungus, was prepurified by fractionated precipitation with ethanol and extracted by reversed micelles of N-benzyl-N-dodecyl-N-bis(2-hydroxyethyl) ammonium chloride (BDBAC) cationic surfactant. A 25-1 fractional factorial design was employed to evaluate the influence of the following factors on the enzyme extraction: pH
(A), conductivity (B), surfactant concentration (C), cosolvent concentration (D) and temperature (E). A statistical analysis
of the results revealed that, of the five variables studied, pH, surfactant concentration, and temperature had significant
main effects (p<0.05) on the recovery of β-xylosidase (Y). However, the interactions between pH and temperature, conductivity and cosolvent
concentration, conductivity and temperature, BDBAC concentration and temperature also had significant influences. A first-order
model (R
2=0.98) expressed by the equation Y=7.73−5.55A−0.67B+3.49C−0.41D+5.26E+2.05AB−3.26AC+0.96AD−7.07AE−3.93BD−2.19BE+0.99CD+0.78CE
was proposed to represent the enzyme recovery as a function of the effects that were really significant. This model predicts
a recovery value of about 40%, which is similar to that obtained experimentally (39.5%). |
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Keywords: | Reverse micelles liquid-liquid extraction β -xylosidase cationic surfactant downstream processing |
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