Spontaneous and facilitated micelles formation at liquid|liquid interface: towards amperometric detection of redox inactive proteins |
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| Institution: | 1. CSIRO Manufacturing, 343 Royal Parade, Parkville, 3052, Australia;2. Department of Microbiology and Immunology, The University of Melbourne at The Peter Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne, 3000, Australia;3. The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, 30 Royal Parade, Parkville, 3052, Australia;4. Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia, V6T 1Z1, Canada;1. Institute of Biochemistry, Vilnius University, Mokslininku 12, LT-08662 Vilnius, Lithuania;2. Institut de Chimie et des Matériaux Paris-Est, Université Paris-Est, 2 rue Henri Dunant, 94320 Thiais, France;1. Laboratório Integrado de Morfologia, Núcleo em Ecologia e Desenvolvimento Sócio Ambiental de Macaé – NUPEM, Universidade Federal do Rio de Janeiro – UFRJ, Macaé, RJ, Brazil;2. Laboratório Integrado de Bioquímica Hatisaburo Masuda, Núcleo em Ecologia e Desenvolvimento Sócio Ambiental de Macaé – NUPEM, Universidade Federal do Rio de Janeiro – UFRJ, Macaé, RJ, Brazil;3. Laboratório de Neurobiologia Comparativa e do Desenvolvimento, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro – UFRJ, Rio de Janeiro, RJ, Brazil |
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| Abstract: | Spontaneous micelles formation by ionic surfactants has been detected amperometrically as an appearance of ion transfer across the water–dichloroethane interface noticed from linear dependence between the current and potential (Ohm’s law). At low surfactant concentrations, when its spontaneous aggregation does not occur, the micelles formation facilitated by a potential across the interface has been registered. The transfer of redox inactive proteins through water–dichloroethane interface in the presence of surfactant has been observed voltammetrically. It has been shown, that the presence of protein does not affect thermodynamics of micelles formation, but accelerates kinetics of ion transfer through the interface. The electrochemically controlled transfer of redox inactive proteins through liquid|liquid interface may lead to the development of methods for direct amperometric detection of biomolecules. |
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