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In situ formation and scanning electrochemical microscopy assisted positioning of NO-sensors above human umbilical vein endothelial cells for the detection of nitric oxide release
Institution:1. Laboratoire d’Electrochimie et de Chimie Analytique (UMR CNRS-ENSCP No. 7575), Ecole Nationale Supérieure de Chimie de Paris, 11 Rue Pierre et Marie Curie, 75231 Paris Cedex 05, France;2. Lehrstuhl für Analytische Chemie – Elektroanalytik und Sensorik, Ruhr-Universität Bochum, Universitätsstr 150, D-44780 Bochum, Germany;1. Department of Materials Science and Technology, Faculty of Engineering, Niigata University, 8050 Ikarashi-2, Niigata 950-2181, Japan;2. Department of Material and Biological Chemistry, Faculty of Science, Yamagata University, 1-4-12 Kojirakawa-machi, Yamagata 990-8560, Japan;1. Sorbonne Université, Faculté de Sciences et Ingénierie, CNRS, Laboratoire de Réactivité de Surface (LRS, UMR 7197), 4 place Jussieu, (case courrier 178), F-75005 Paris, France;2. Sorbonne Université, Faculté de Sciences et Ingénierie, CNRS, Laboratoire Interfaces et Systèmes Electrochimiques (LISE, UMR 8235), 4 place Jussieu, (case courrier 133), F-75005 Paris, France;3. Department of Chemical Engineering, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan;1. CNRS, UMR 8235, Laboratoire Interfaces et Systèmes Electrochimiques, 4, place Jussieu, F-75005, Paris, France;2. Sorbonne Universités, UPMC Univ Paris 06, UMR 8235, LISE, 4, place Jussieu, F-75005, Paris, France;1. Vilnius University, Faculty of Chemistry and Geosciences, Institute of Chemistry, Naugarduko 24, Vilnius, LT-03225, Lithuania;2. Laboratory of Nanotechnology, State Research Institute Centre of Physical Sciences and Technology, Sauletekio ave. 3, LT-10257, Vilnius, Lithuania
Abstract:Pt microelectrodes (50 μm diameter) were positioned by means of scanning electrochemical microscopy assisted z-approach curves and in situ modified with nickel tetrasulfonated phthalocyanine tetrasodium salt as electrocatalytic layer for the specific oxidative detection of nitric oxide. The thus modified electrodes were then moved over a layer of adherently growing human umbilical vein endothelial cells (HUVEC) in order to amperometrically detect nitric oxide (NO) released from the cells upon stimulation with bradykinin. This approach actually takes advantage of the use of SECM to define a sequential procedure that enables the in situ functionalisation of the SECM tip thus allowing to accurately control the separation between the functionalised SECM tip and the cell population.
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