Excretion of iodide in 24-h urine as determined by ion-pair reversed-phase liquid chromatography with electrochemical detection |
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Authors: | J Odink J J Bogaards H Sandman R J Egger G A Arkesteyn P de Jong |
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Institution: | Department of Clinical Biochemistry, TNO-CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands. |
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Abstract: | A simple method is presented for the routine analysis of iodide in urine. After a one-step sample clean-up, iodide was separated by ion-pair reversed-phase liquid chromatography and detected electrochemically with a silver electrode. The coefficient of variation of a single analysis of iodide in a pooled urine sample (530 nmol/l) was 7.6%. The detection limit, derived from a signal-to-noise ratio of 3, was 3 pmol, corresponding to 0.06 mumol/l. The recovery of iodide added to urine was 96 +/- 7%. The accuracy of the method was assessed by analysing ten different samples with neutron activation analysis. The data obtained with the two methods showed a high correlation (r = 0.991) and did not differ significantly. Excretion of iodide in samples of 24-h urine from a free-living population was shown to have a log-normal distribution and to be higher in men than in women. The iodide/creatinine ratio was independent of sex and increased with age. |
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