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Direct MALDI-MS Analysis of Proteins Isolated by Liquidphase Isoelectric Focusing Electrophoresis |
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| Authors: | LIU Ning ZHANG Xuan WANG Zhi SUN Ya-dong LIU Zhi-qiang LIU Shu-ying |
| Abstract: | A liquid-phase isoelectric focusing electrophoresis system(Rotofor) was used as the prefractionation tool for the sample preparation in the MALDI-MS analysis of a protein mixture. Each fraction collected was then directly subjected to MALDI-TOF-MS analysis. By this approach, we are able to resolve two types of hemoglobins, A and C, which cannot be successfully separated by means of the traditional SDS-PAGE method. |
| Keywords: | Liquid-phase isoelectric focusing electrophoresis MALDI-MS analysis Hemoglobin A and C Cytochrome c |
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