带羧基单分散彩色微球的制备

采用两步活性溶胀种子聚合法, 制备了可用于免疫检测的3种不同颜色的表面带有羧基功能基的粒径在400—800 nm之间的彩色单分散微球. 先用无皂乳液聚合法制备出单分散聚苯乙烯种子, 然后用邻苯二甲酸二正丁酯(DBP)作为溶胀剂对微球进行溶胀, 溶涨后的种子模板再用混溶的苯乙烯、二乙烯苯、丙烯酸、双键彩色染料以及引发剂(BPO)溶胀, 升温聚合后得到理想的单分散微球. 考察了DBP和单体用量、各单体配比及染料对微球的形貌和单分散性的影响.

Preparation of Carboxyl Monodispersed Colour Microspheres

Monodispersed polystyrene microspheres with three distinct dyes and carboxyl group in the size range of 400—800 nm were prepared with seed polymerization and swelling, which affords a new method to combine the dyes with the microspheres. Monodispersed polystyrene seed was produced via emulsifier-free emulsion polymerization. It was swollen first by di-n-butyl phthalate(DBP), then by the mixture of styrene(St)-acrylic acid(AA)-divinylbenzene(DVB)-dyes including an oil-soluble initiator. The final microspheres were obtained via the polymerization of monomer mixture in the swollen seed microspheres at 70 ℃. The average size, size distribution and surface morphology of the final beads were characterized via SEM. The effects of the quantity of DBP, monomer and monomer proportion on the size distribution and surface morphology were discussed.