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Fast C18 solid-phase desalting/delipidation of the human serum apolipoproteins for matrix-assisted laser desorption ionization and electrospray ionization mass spectrometric analysis |
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| Authors: | Layle K Watkins Pavel V Bondarenko Damon C Barbacci Siqing Song Steven L Cockrill David H Russell Ronald D Macfarlane |
| Institution: | a Texas A&M University, Department of Chemistry, P.O. Box 300012, College Station, TX 77842-3012, USA b Thermo BioAnalysis Corporation, 504 Airport Road, P.O. Box 2108, Santa Fe, NM 87504-2108, USA c Centers for Disease Control and Prevention, MS F19, Atlanta, GA 30345, USA |
| Abstract: | A new method for the delipidation of human serum lipoproteins involving the use of a reversed-phase C18 solid-phase extraction (SPE) cartridge is introduced for use with matrix-assisted laser desorption ionization and electrospray ionization mass spectrometry. This method is compared with two other methods of lipoprotein delipidation. The SPE method of delipidation produces a higher and more reproducible protein yield than the conventional liquid–liquid methanol–diethyl ether delipidation technique. Furthermore, the SPE method implements a fast, sequential, desalting and delipidation of the lipoproteins for subsequent mass spectrometric analysis providing high quality spectra. |
| Keywords: | Apolipoproteins Lipoproteins Proteins |
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