Effects of sodium phosphate buffer on horseradish peroxidase thermal stability |
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Authors: | L Haifeng L Yuwen C Xiaomin W Zhiyong W Cunxin |
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Institution: | (1) College of Chemistry and Molecular Science, Wuhan University, Wuhan, Hubei, 430072, P.R. China |
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Abstract: | Thermal stability of horseradish peroxidase (HRP) was studied by differential scanning calorimetry, tryptophan fluorescence,
the heme absorption and enzymatic activity analysis while the concentrations of sodium phosphate buffer ranged from 2.5 to
50 mM at pH 7.0.
The results showed that the denaturation temperature (T
m) values decreased and the intrinsic tryptophan fluorescence intensity of denatured HRP increased as sodium phosphate buffer
concentration increased. Furthermore, the heme absorbance at 403 nm and enzymatic activity of HRP decreased with the increasing
buffer concentrations. According to data obtained in this experiment, it can be concluded that sodium phosphate accelerated
the denaturation process of HRP and reduced the thermal stability of HRP. |
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Keywords: | DSC enzymatic activity fluorescence heme absorption horseradish peroxidase sodium phosphate buffer thermal stability |
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