邻羟基苯甲酸分子印迹聚合物对于异构体的识别及色谱行为研究

采用分子印迹技术，以邻羟基苯甲酸（水杨酸）为模板分子，α-甲基丙烯酸 为功能单体，采用不同的交联剂和致孔剂，合成了用于分离羟基苯甲酸异构体的印 迹聚合物，并以高效液相色谱及平衡吸附的方法对聚合物进行了评价。实验结果表 明，以氢键为主要结合作用力的水杨酸印迹聚合物具有特定的空间识别位点，在弱 极性溶剂中对模板分子有较强的选择性，可以对官能团位置异构体进行很好地分离 。研究了以氢键为主要作用力所形成的分子印迹聚合物在色谱分离中的应用，并讨 论了流动相对分离的影响及印迹聚合物和客体分子的作用机制。

Study on the Isomer Recognition and Chromatographic Behaviors of Salicylic Acid Imprinted Polymers

Molecularly imprinted polymers were synthesized with salicylic acid as the template, methacrylic acid as the functional monomer to study the binding property of the polymer which utilizing hydrogen bonding as the major interaction force. Results of chromatographic and adsorptive isotherm studies show that imprinted polymer has specific binding affinity for salicylic acid. Scatchard analysis shows that different binding classes formed in the polymer. The polymer selectivity was indicated by the separation of salicylic acid and its isomer: 4 hydroxybenzoic acid in the HPLC analysis with the resulting polymer as a stationary phase. Influences of the mobile phase on the HPLC analysis and recognition mechanism were also discussed.