Desulfurization of dibenzothiophene, benzothiophene, and other thiophene analogs by a newly isolated bacterium, Gordonia alkanivorans strain 1B |
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Authors: | Luís Alves Rita Salgueiro Carla Rodrigues Elsa Mesquita José Matos Francisco M Gírio |
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Institution: | 1. Tecnologia Inovacáo, Departmento de Biotecnologia, Instituto Nacional de Engenharia, Estrada do Pa?o do Lumiar, 22, 1649-038, Lisboa, Portugal
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Abstract: | A novel bacterium, Gordonia alkanivorans strain 1B, was isolated from hydrocarbon-contaminated soil. Assessment of the biodegradation of distinct organic sulfur-compounds,
such as dibenzothiophene (DBT), benzothiophene (BT), DBT sulfone, and alkylated tiophenic compounds, as the sole source of
sulfure was investigated. G. alkanivorans strain 1B was able to remove selectively the sulfur from DBT while keeping intact the remaining carbon-carbon structure.
Orthophenyl phenol (2-hydroxybiphenyl) was the only detected metabolic product. The bacterial desulfurization activity was
repressed by sulfate. G. alkanivorans straini 1B consumed 310 μM DBT after 120 h of cultivation, corresponding to a specific desulfurization rate of 1.03 μmol/(g of dry cells·h). When an
equimolar mixture of DBT/BT was used as a source of sulfur in the growth medium, G. alkanivorans strain 1B assimilated both compounds in a sequential manner, with BT as the preferred source of sulfur. Only when BT concentration
was decreased to a very low level was DBT utilized as the source of sulfur for bacterial growth. Thespecific desulfurization
overall rates of BT and DBT obtained were 0.954 and 0.813 μmol/(g of dry cells·h), respectively. The newly isolated G. alkanivorans strain 1B has good potential for application in the biodesulfurization of fossil fuels. |
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Keywords: | Biodeusulfurization dibenzothiophene benzothiophene sulfur Gordonia alkanivorans |
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