Tb3+离子与植物辣根过氧化物酶的作用方式探讨 |
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引用本文: | 亓昭鹏,周青,杜江燕,陆天虹,李邨,陈蕴,黄晓华. Tb3+离子与植物辣根过氧化物酶的作用方式探讨[J]. 化学学报, 2005, 63(1): 1-4 |
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作者姓名: | 亓昭鹏 周青 杜江燕 陆天虹 李邨 陈蕴 黄晓华 |
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作者单位: | (南京师范大学化学与环境科学学院 南京 210097);(江南大学工业生物技术教育部重点实验室 无锡 214036);(中国科学院长春应用化学研究所 长春 130022) |
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摘 要: | 利用紫外可见吸收光谱、红外光谱、荧光光谱、原子吸收及酶分离方法, 首次研究了稀土离子Tb3+与植物辣根过氧化物酶(HRP)的相互作用方式. 结果表明, Tb3+与HRP作用方式包括: (1) Tb3+与肽链上的氨基酸残基作用, 影响酶活性中心的微结构; (2) Tb3+能部分取代酶中的Ca2+; (3) Tb3+能部分剪切肽链上的氨基酸残基, 改变酶的结构. 因此, Tb3+与HRP的相互作用可能以一种方式为主, 或几种作用方式同时存在.
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关 键 词: | 稀土铽离子 辣根过氧化物酶 酶活性 作用方式 |
收稿时间: | 2004-04-13 |
修稿时间: | 2004-10-25 |
Study on Interaction Ways between Tb3+and Horseradish Peroxidase |
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Affiliation: | (Department of Chemistry, Nanjing Normal University, Nanjing 210097)(Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036)(Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022) |
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Abstract: | The interaction ways between Tb3+and horse radish peroxidase (HRP) were investigated for the first time using UV-Vis absorption spectroscopy, fluorescence spectroscopy, atomic absorption spectroscopy and enzyme separation technique. The experimental results indicated that there are three interaction ways between Tb3+and HRP. Firstly, Tb3+would bind to N or O atoms in the polypeptide of HRP. It would affect the conformation of HRP. Secondly, Tb3+can replace Ca2+partially. Thirdly, Tb3+would shear the polypeptide and thus change the structure of HRP. Therefore, Tb3+may interact with HRP in one way mainly or three ways simultaneously. |
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Keywords: | Tb3+ horseradish peroxidase enzyme activity interaction way |
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