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Size-exclusion chromatographic behavior of bovine serum albumin in uni-uni valent ions solutions |
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| Authors: | S. F. Sun S. Del Terzo R. A. Nash |
| Affiliation: | (1) Department of Chemistry, St. John's University, 11439 Jamaica, New York, (USA);(2) Department of Industrial Pharmacy, College of Pharmacy and Allied Health, St. John's University, 11439 Jamaica, New York, (USA) |
| Abstract: | Summary Molecular interaction between uni-uni valent ions and bovine serum albumin was investigated by size-exclusion chromatography. Elution profiles are first presented for salt and protein solutions as samples with water as the mobile phase; then for water and protein as samples with salt solutions as the mobile phase. The results suggest the existence of a dynamic equilibrium between the salt ions and the protein ions as reactants and the ion-pair (salt-protein) complex as a product. |
| Keywords: | Column liquid chromatography Size-exclusion Dynamic equilibrium Ion-pairing interaction Monomer-dimer separation |
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