皂苷的电喷雾负离子多级串联质谱研究

皂苷作为一类结构比较复杂的糖苷类化合物 ,广泛地存在于植物和海洋生物中 .现代医学研究表明 ,皂苷类化合物具有十分广泛的药理作用 .目前 ,在我国许多以皂苷类化合物为主要有效成分的药物都具有很好的疗效 .因此研究人员对于皂苷类化合物的结构研究有着愈来愈浓厚的兴趣 .然而皂苷类化合物具有极性大、难挥发、分子量大等特点 ,给分离纯化及结构鉴定带来很大的困难 .对于这类化合物结构的研究远远落后于生物碱和黄酮 .目前采用质谱技术测定皂苷类化合物的结构已有文献报道 ,但多局限于正离子质谱[1～ 6] ,对于负离子质谱仅限于 MS/ MS质…

Studies on Negative Electrospray Ionization Mass Spectrometry on Saponins

Two kinds of saponins have been investigated by negative electrospray ionization(NESI) mass spectrometry. Under ESI conditions, the - ions of saponins were observed which provide the molecular weights of saponins. The fragment pathways of - ions of these two saponins depend on their structures. For steroidic saponins, - ion only produces the fragment ions by the losses of sugar units. For oleanolic saponins, - ion yields the cross ring ions as well as the fragment ions by the losses of sugar units. Moreover, the abundance of the former is higher than that of the latter. The characteristic fragments are used to provide the sequence and some linkage information of sugar moieties of saponins. Especially, their fragment difference strongly depends on the linkage between the aglycone and the sugar moieties.