| 3种色谱模式联用在中药活性成分初步筛选中的应用 |
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| 引用本文： | 毛希琴,邹汉法,封顺,孔亮,黄晓冬,厉欣,罗权舟.3种色谱模式联用在中药活性成分初步筛选中的应用[J].分析化学,2003,31(8):992-995. |
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| 作者姓名： | 毛希琴 邹汉法 封顺 孔亮 黄晓冬 厉欣 罗权舟 |
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| 作者单位： | 中国科学院大连化学物理研究所,国家色谱研究分析中心,大连,116021 |
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| 基金项目： | 国家杰出青年基金 (No .2 972 5 5 12 )，中国科学院生物局特别支持课题 (STZ 0 0 0 9)资助项目 |
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| 摘 要： | 提出了反相高效液相色谱(RP-HPLC)、固定化脂质体色谱(ILC)以及固定化载体蛋白色谱(ICPC)3种色谱模式联用筛选中药中活性成分的新思路，并用于传统中药川芎中的生物活性成分的初步筛选。从川芎的甲醇提取液中筛选出几种既有细胞膜的穿透能力又有与载体蛋白的结合能力的成分，并对其中两种主要的组分进行了初步的结构鉴定。
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| 关 键 词： | 色谱模式联用 中药 活性成分 筛选 固定化脂质体色谱 载体蛋白 川芎 液相色谱 分离 |
Combining Three Chromatographic Modes for Screening Bioavailable Components in Chinese Medicines |
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| Abstract: | A new method combining three chromatographic modes, reverse phase high performance liquid chromatography (RP-HPLC), immobilized liposome chromatography (ILC) and immobilized carrier protein chromatography (ICPC) to screen bioavailable components in traditional Chinese medicines (TCMs) was proposed and applied in screening of bioavailable components in Ligusticum Wallichii, a traditional Chinese medicine. Several possibly bioavailable components being of permeability through cell membrane as well as binding ability with carrier protein were separated and two of them were preliminarily identified. |
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| Keywords: | Liquid chromatography immobilized liposome chromatography carrier protein Chinese medicine ligusticum wallichii bioavailable component |
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