| 光谱法研究铜锌超氧歧化酶与组氨酸钴（Ⅱ）的相互作用：Ⅲ. … |
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| 引用本文： | 郑学仿 胡皆汉. 光谱法研究铜锌超氧歧化酶与组氨酸钴（Ⅱ）的相互作用：Ⅲ. …[J]. 光谱学与光谱分析, 1999, 19(6): 803-805 |
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| 作者姓名： | 郑学仿 胡皆汉 |
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| 摘 要： | 采用ＶＩＳ，ＩＣＰ及酶活性测定等方法，研究铜锌超氧歧化酶（Ｃｕ－Ｚｎ２ＳＯＤ）与组氨酸钴（Ⅱ）（Ｃｏ（ＨｉＳ）。直接相互作用时溶液ｐＨ值、作用时间对此类相互作用的影响。结果发现，溶液的ｐＨ值越高，作用时间越长，超有利于类此相互作用的进行。
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| 关 键 词： | 铜锌超氧歧化酶 组氨酸钴 相互作用 SOD |
Study on the interaction of copper-zinc superoxide dismutase with cobalt (II)-histidine by spectral analysis. II. Effects of pH, interaction time] |
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| X Zheng,J Hu,G Cheng,Y Xu,Y Zhao,M Liu. Study on the interaction of copper-zinc superoxide dismutase with cobalt (II)-histidine by spectral analysis. II. Effects of pH, interaction time][J]. Spectroscopy and Spectral Analysis, 1999, 19(6): 803-805 |
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| Authors: | X Zheng J Hu G Cheng Y Xu Y Zhao M Liu |
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| Affiliation: | Dalian Institute of Chemical Physics, Academia Sinica, 116023 Dalian. |
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| Abstract: | The direct interaction of Cu2Zn2SOD with organic metal compound (Cobalt (II)-Histidine) was studied by ICP, VIS and the measurement of enzyme activity, and also investigated the effect of pH values and different interaction times for this interaction. The results showed that with the increased in pH values and the more longer interaction times, the intensity of interaction were increased and the corresponding catalytic activity of enzyme were affected. |
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