| Site-specifically cleavage of oxidized insulin B chain with Zn（Ⅱ） ion studied by electrospray ionization mass spectrometry |
| |
| 作者姓名： | Jiang Jiang Yu Hua Mei Long Gen Zhu Wen Ji Wang |
| |
| 作者单位： | [1]College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, Chin [2]College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China [3]State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China |
| |
| 摘 要： | The electrospray ionization mass spectrometry and tandem mass spectrometry investigation showed that the binding sites of Zn^2＋ with oxidized insulin B chain are His 5, His 10, and Arg 22, which lead to the selective cleavages of the peptide bonds at Ash 3- Gin 4, His 5-Leu 6, Gly 8-Ser 9, and Glu 21-Arg 22 of oxidized insulin B chain.
|
| 关 键 词： | 氧化胰岛素 B链 Zn（Ⅱ）离子 位点特异性分裂 电喷雾离子化质谱法 |
| 收稿时间： | 2006-11-13 |
Site-specifically cleavage of oxidized insulin B chain with Zn(II) ion studied by electrospray ionization mass spectrometry |
| |
| Jiang Jiang Yu Hua Mei Long Gen Zhu Wen Ji Wang.Site-specifically cleavage of oxidized insulin B chain with Zn(II) ion studied by electrospray ionization mass spectrometry[J].Chinese Chemical Letters,2007,18(5):557-560. |
| |
| Authors: | Jiang Jiang Yu Hua Mei Long Gen Zhu Wen Ji Wang |
| |
| Institution: | 1. College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China;2. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;3. State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China |
| |
| Abstract: | The electrospray ionization mass spectrometry and tandem mass spectrometry investigation showed that the binding sites of Zn2 with oxidized insulin B chain are His 5, His 10, and Arg 22, which lead to the selective cleavages of the peptide bonds at Asn 3-Gln 4, His 5-Leu 6, Gly 8-Ser 9, and Glu 21-Arg 22 of oxidized insulin B chain. |
| |
| Keywords: | Oxidized insulin B chain ZnCl2 Mass spectrometry Hydrolytic cleavage |
| 本文献已被 CNKI 维普 ScienceDirect 等数据库收录！ |
