| 聚乙烯基酯树脂整体柱的制备及其对免疫球蛋白的分离 |
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| 引用本文： | 杨冠群a,杨更亮a,b,刘海燕a,白立改a,封晓娟a,杨欣茹a.聚乙烯基酯树脂整体柱的制备及其对免疫球蛋白的分离[J].中国化学,2009,27(4):777-780. |
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| 作者姓名： | 杨冠群a 杨更亮a b 刘海燕a 白立改a 封晓娟a 杨欣茹a |
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| 作者单位： | a 河北大学药学院，河北省药物质量研究重点实验室，河北保定，071002，中国 ;b 中国科学院化学研究所，北京，100080，中国 ; |
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| 摘 要： | 以一种新型的材料聚乙烯基酯树脂（甲基丙烯酸间苯二酚二缩甘油酯）同时为单体和交联剂，以偶氮二异丁基腈（AIBN）为引发剂，以十六醇为制孔剂制备所得的聚合物整体柱，经化学修饰后，考察其作为HPLC的固定相对免疫球蛋白等进行分离，结果表明分离效果良好，适合作为HPLC的固定相。
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| 关 键 词： | 免疫球蛋白 高效液相色谱 聚乙烯基酯树脂 整体柱 |
| 收稿时间： | 2008-8-24 |
| 修稿时间： | 2008-12-12 |
Separation of Immunoglobulin in Conjunction with High Performance Liquid Chromatography Using Poly(vinyl ester resin) Monolith |
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| Guanqun YANG,Gengliang YANG,Haiyan LIU,Ligai BAI,Xiaojuan FENG,Xinru YANG.Separation of Immunoglobulin in Conjunction with High Performance Liquid Chromatography Using Poly(vinyl ester resin) Monolith[J].Chinese Journal of Chemistry,2009,27(4):777-780. |
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| Authors: | Guanqun YANG Gengliang YANG Haiyan LIU Ligai BAI Xiaojuan FENG Xinru YANG |
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| Institution: | 1. College of Pharmacy and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, Baoding, Hebei 071002, China;2. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China;3. Tel.: 0086‐312‐5079788;4. Fax: 0086‐312‐5971107 |
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| Abstract: | Using vinyl ester resin both as the monomer and the crosslinker, 2,2‐azobis(2‐methylpropionitrile) (AIBN) as initiator, poly(vinyl ester resin) monolithic column was prepared to separate the immunoglobulin G (IgG) and yolk of eggs (IgY) from human plasma and egg yolk by HPLC respectively. The influence of concentration and pH of mobile phase on the chromatographic performance was investigated. Scanning electron microcopy showed that the poly(vinyl ester resin) monolithic stationary phase had a through‐pore structure. Dynamic capacity of IgG on the polymeric monolithic column was investigated. The monolithic column had good flow‐through properties and high resolution. The maximum adsorptive amount of IgG was 144 µg·g?1. |
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| Keywords: | immunoglobulin HPLC poly(vinyl ester resin) monolith |
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