Synthesis of alpha-L-threofuranosyl nucleoside triphosphates (tNTPs) |
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Authors: | Zou Keyong Horhota Allen Yu Biao Szostak Jack W McLaughlin Larry W |
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Affiliation: | Howard Hughes Medical Institute and Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02184, USA. |
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Abstract: | structure: see text] The alpha-l-threofuranosyl nucleoside triphosphates of T, G, and D (tTTP, tGTP, and tDTP) were synthesized from the described 2'-O-DMT-protected derivatives using the Eckstein method, while the corresponding C derivative (tCTP) was prepared from the 2'-O-acetyl derivative. The prepared alpha-l-threofuranosyl nucleoside triphosphates, despite being one carbon shorter than the native 2'-deoxyfuranosyl nucleoside triphosphates, are effective substrates for selected DNA polymerases. |
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