比率荧光纳米水凝胶的制备以及在pH探测中的应用

基于再沉淀方法制备了一种具有比率荧光发射的纳米水凝胶,适用于检测生理范围的pH值。通过在聚氨酯水凝胶中引入pH值指示剂以及具有共振能量传递关系的荧光染料分子,赋予原本非pH响应和非荧光型的水凝胶以pH探测能力。随着pH值由酸性渐变为碱性,纳米水凝胶的绿色荧光强度逐渐增强,而红色荧光逐渐减弱。所制备的纳米水凝胶由于具有灵敏度高、亲水性好、稳定性好、响应快以及pH值检测范围恰好涵盖了生理pH值范围(pH值6～8)等优点,因此在细胞内pH值探测领域具有广阔应用前景。

A Ratiometric Fluorescense Nanogel for pH Sensor

In this paper a ratiometric fluorescence nanogel was prepared and used in pH sensing. By introducing a pH probe and two fluorescent dyes (related by fluorescent resonant energy transfer), the non-responsive and non-fluorescent hydrogel (polyurathane) was imparted the ability of pH sensing. With the increase of pH value from acidic to basic, the dominant fluorescence of nanogels varieds from red to green accordingly. The as-prepared nanogels exhibits high sensitivity, high hydrophilicity, quick responsiveness, and physiological pH detecting in the range of 6~8. Hence, the nanogels would be very potential as intracellular pH nanosensors.