Preparation of a novel composite particles and its application in the fluorescent detection of proteins |
| |
Authors: | Hong-Qi Chen Lun Wang Yan Liu Wei-Li Wu A-Ni Liang Xiao-Lei Zhang |
| |
Institution: | (1) College of Chemistry and Material Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, Wuhu, 241000, People’s Republic of China |
| |
Abstract: | A new fluorescence method for the detection of proteins with novel composite nanoparticles (CdS/PPA) has been developed. The
composite nanoparticles have been prepared through an in-situ polymerization method under ultrasonic irradiation. The surface
of the composite nanoparticles was covered with functional groups (-COOH). These groups may play a major role in the improving
the water solubility and biocompatibility of the nanoparticles. The composite particles is combined with proteins in NaAc-HCl
buffer solution (pH=1.99), which can result in strong fluorescence, and the response is linearly proportional to the concentration
of proteins. In λem/λex=650 nm/365 nm place (the stoke’ shift is 285 nm), its fluorescent strength reaches the maximum. Under
the optimum conditions, the linear range is 0.10–20.0 μg·ml−1 with the detection limit of 41 ng·ml−1 for HSA, and 0.10–15.0 μg·ml−1 with the detection limit of 35 ng·ml−1 for Human γ-IgG . The method has been applied to the determination of the total protein in human serum samples collected
from the hospital and the results are satisfactory. |
| |
Keywords: | Synthesis Composite nanoparticles Fluorescence assay Protein Detection |
本文献已被 PubMed SpringerLink 等数据库收录! |
|