Immobilized metal-ion affinity chromatography of peptides on metalloporphyrin stationary phases |
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Authors: | Magdalena Biesaga Jowita Orska Dariusz Fiertek J Izdebski M Trojanowicz |
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Institution: | (1) Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland e-mail: trojan@chem.uw.edu.pl, PL |
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Abstract: | The retention of selected dipeptides and tripeptides containing tyrosine was examined. As stationary phase an aminopropylated
silica gel loaded with covalently linked tetraphenylporphyrin was used. The effect of metalization of porphyrin with Cu(II)
and Zn(II) on retention was investigated. The observed separation is based on a mixed mechanism involving π-π and hydrophobic
interactions as well as complex formation between immobilized metal ions and peptides. A satisfactory separation was demonstrated
for C-peptide and bovine insulin. The possibility of separation of various insulins was also investigated.
Received: 10 August 1998 / Revised: 21 December 1998 / Accepted: 28 December 1998 |
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