Enzymatic saccharification and fermentation of xylose-optimized dilute acid-treated lignocellulosics |
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| Authors: | Yun-Chin Chung Alan Bakalinsky Michael H Penner |
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| Institution: | (1) Department of Food and Nutrition, Providence University, Taichun, Taiwan;(2) Department of Food Science and Technology, Oregon State University, Corvallis, OR, USA |
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| Abstract: | The cellulose reactivity of two lignocellulosic feedstocks, switchgrass and poplar, was evaluated under straight saccharification
(SS) and simultaneous saccharification and fermentation (SSF) conditions following dilute sulfuric acid pretreatments designed
for optimum xylose yields. The optimum pretreatment conditions, within the constraints of the experimental system (Parr batch
reactor), were 1.2% acid, 180°C, and 0.5 min for switchgrass and 1% acid, 180°C, and 0.56 min for poplar. The cellulase enzyme
preparation was from Trichoderma reesei and fermentations were done with Saccharomyces cerevisiae. Time courses for SS were monitored as the sum of glucose and cellobiose; those for SSF as the sum of glucose, cellobiose,
and ethanol. Percentage conversions under SS conditions were 79.1% and 91.4% for the pretreated poplar and switchgrass feedstocks,
respectively. Analogous values under SSF conditions were 73.0% and 90.3% for pretreated poplar and switchgrass, respectively. |
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| Keywords: | Lignocellulosic poplar switchgrass corn stover simultaneous saccharification and fermentation cellulase cellulose xylose |
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