1. Department of Biomedical Science, CHA University, 502 Yatop‐dong, Bundang‐gu, Seongnam‐si, Gyeonggi‐do 463‐840, Korea;2. Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31 Hyoja‐dong Nam‐gu, Pohang 790‐784, Korea;3. Department of Neurosurgery, CHA University, Bundang CHA Medical Center, Yatap‐dong Bungdang‐gu, Seongnam‐si, Gyeonggi‐do 463‐712, Korea;4. Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), San 31 Hyoja‐dong Nam‐gu, Pohang 790‐784, Korea
Abstract:
In this study, we report an efficient and cost‐effective method of fabricating polystyrene (PS) nano‐featured substrates containing nanopore (NPo) and nanopillar (NPi) arrays based on hot embossing using nickel nano‐stamps. We investigate the behavior of adipose‐derived stem cells (ASCs), including adhesion, morphology, proliferation and differentiation, on the replicated PS surfaces. Compared to a flat substrate, NPo‐ and NPi‐featured substrates do not alter the morphology of stem cells. However, both NPo‐ and NPi‐featured substrates induce different integrin expression and lower formation of focal adhesion complexes. In addition, ASCs on the NPo‐featured substrate exhibit greater adipogenic differentiation, while the NPi‐featured substrate induces higher osteogenic differentiation.
