Urinary metabonomic study using a CUMS rat model of depression |
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| Authors: | Xiao‐Jie Liu Zhen‐Yu Li Zhong‐Feng Li Xiao‐Xia Gao Yu‐Zhi Zhou Hai‐Feng Sun Li‐Zeng Zhang Xiao‐Qing Guo Guan‐Hua Du Xue‐Mei Qin |
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| Affiliation: | 1. Modern Research Center for Traditional Chinese Medicine of Shanxi University, , Taiyuan, China;2. College of Chemistry and Chemical Engineering of Shanxi University, , Taiyuan, China;3. Department of Chemistry, Capital Normal University, , Beijing, China;4. Chinese Academy of Medical Sciences, Institute of Materia Medica, , Beijing, China |
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| Abstract: | Chronic unpredictable mild stress (CUMS) is a well‐validated model of depression. In this study, a urinary metabonomics method based on the NMR spectrometry was used to study the metabolic perturbation in CUMS‐induced rat depression model. With pattern recognition analysis, a clear separation of CUMS rats and healthy controls was achieved, and nine endogenous metabolites contributing to the separation were identified. CUMS‐treated rats were characterized by the increase of glycine, pyruvate, glutamine, and asparagines, as well as the decrease of 2‐oxoglutarate, dimethylglycine, citrate, succinate, and acetate. The urinary biochemical changes related to the metabolic disturbance in CUMS induced depression, and the possible correlations with live qi stagnation in traditional Chinese medicine are discussed. The work shows that CUMS is a reliable model for studying depression, and the noninvasive urinary metabolomic method is a valuable tool to investigate the biochemical pertubations in depression as an early diagnostic means. Copyright © 2012 John Wiley & Sons, Ltd. |
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| Keywords: | NMR 1H metabonomics depression urine CUMS |
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