Preparative isolation and purification of harpagoside and angroside C from the root of Scrophularia ningpoensis Hemsley by high‐speed counter‐current chromatography |
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Authors: | Jinfeng Tian Xiaoli Ye Yuanhong Shang Yafei Deng Kai He Xuegang Li |
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Institution: | 1. Chemistry Institute of Pharmaceutical Resources, College of Pharmaceutical Sciences, Chongqing Engineering Center of Pharmacodynamics Evaluation, Southwest University, , Chongqing, China;2. School of Life Science, Southwest University, , Chongqing, China |
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Abstract: | In this study, the bioactive component harpagoside and angroside C in the root of Scrophularia ningpoensis Hemsley was simultaneously separated by high‐speed counter‐current chromatography (HSCCC). A two‐phase solvent system containing chloroform/n‐butanol/methanol/water (4:1:3:2, v/v/v/v) was selected following consideration of the partition coefficient of the target compound. The crude extract (200 mg) was loaded onto a 280‐mL HSCCC column and yielded 22 mg harpagoside and 31 mg angroside C with the purity of higher than 98 and 98.5%, respectively. It is feasible to isolate active compounds harpagoside and angroside C from S. ningpoensis using HSCCC. |
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Keywords: | Angroside C Harpagoside High‐speed counter‐current hromatography Scrophularia ningpoensis |
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