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Non‐Adhesive Behavior of New Nanostructured PNIPAM Surfaces Towards Specific Neurodegenerative Proteins: Application to Storage and Titration of Tau Proteins |
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| Authors: | Tjasa Vrlinic Dominique Debarnot Gilbert Legeay Arnaud Coudreuse Benaissa El Moualij Willy Zorzi Armand Perret‐Liaudet Isabelle Quadrio Miran Mozetic Fabienne Poncin‐Epaillard |
| Institution: | 1. Institut des Molécules et Matériaux du Mans, Département Polymères, Collo?des et Interfaces, LUNAM Université, UMR Université du Maine – CNRS no. 6283, Avenue Olivier Messiaen, 72085 Le Mans, France;2. CTTM, 20 rue Thalès de Milet 72000 Le Mans, France;3. Institute of Human Histology‐CRPP, Université de Liège, 1, avenue de l'h?pital, Sart Tilman, B‐4000 Liège, Belgium;4. Center Mémoire de Ressources et Recherche, Service de Neurobiologie, Groupement Hospitalier Est du CHU, 56 bd Pinel, 69677 Bron cedex, France;5. Jozef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia |
| Abstract: | New nonfouling tubes are developed and their influence on the adhesion of neuroproteins is studied. The biomarkers are considered as single components (recombinant prion and Tau proteins) or in a solution of native and pathological forms. The samples are stored for 24 h at 4 °C in virgin and treated tubes layered with two different nanostructured coatings based on poly(N‐isopropylacrylamide) with either a positive or a neutral charge, and the protein adhesion is monitored. The recombinant protein with a high pI is repelled from the nanostructured surface that has a negative ζ potential, whereas the recombinant protein with the lower pI is attracted. Furthermore, in the case of complex solutions, neutral nanostructured surfaces are able to retain all amyloid biomarkers.  |
| Keywords: | biomolecules ELISA nanostructuring non‐adhesion plasma treatment |