In vitro growth and activity of chondrocytes on three dimensional polycaprolactone/chitosan scaffolds |
| |
Authors: | Xiaobing Jiang Xiaoling Lu Bo Xiao Ying Wan Yongxiang Zhao |
| |
Institution: | 1. Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, P.R. China;2. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P.R. China;3. The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, P.R. China |
| |
Abstract: | Porous polycaprolactone/chitosan blend scaffolds with various compositional proportions were prepared using a particulate‐leaching method. The pore parameters of resultant scaffolds were found to be mainly modulated by porogen. The compressive mechanical properties and hydrophilicity of scaffolds were examined by measuring their compressive modulus and stress strength as well as swelling index. Selected chondrocytes isolated from articular cartilage of knee joints of rabbits were seeded on these scaffolds, and further in vitro cultured for various periods. The growth and activity of seeded cells were estimated by counting numbers of cells proliferated on scaffolds and measuring the amounts of proteoglycans and type II collagen synthesized by the seeded cells. It was found that some scaffolds composed of proper component ratios and having appropriate pore parameters exhibited promising characteristics for the adhesion and proliferation of seeded cells while maintaining the phenotype and activity of the cells. Copyright © 2010 John Wiley & Sons, Ltd. |
| |
Keywords: | chitosan polycaprolactone porous scaffold chondrocyte growth and activity |
|
|