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Impact of Peptide Micropatterning on Endothelial Cell Actin Remodeling for Cell Alignment under Shear Stress |
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| Authors: | Céline Chollet Reine Bareille Murielle Rémy Alain Guignandon Laurence Bordenave Gaetan Laroche Marie‐Christine Durrieu |
| Institution: | 1. Bioingénierie Tissulaire, Université Bordeaux, U1026, F‐33000 Bordeaux, France, and Bioingénierie Tissulaire, INSERM, U1026, F‐33000 Bordeaux, France;2. INSERM U1059, Université Jean Monnet, 15 rue Ambroise PARE, St‐Etienne F‐42023, France, and Université Lyon at St‐Etienne, F‐42023, 15 rue Ambroise PARE, F‐42023 St‐Etienne, Cedex 2, France;3. CHU de Bordeaux, CIC‐IT BioDiMI, F‐33000 Bordeaux, France;4. Laboratoire d'Ingénierie de Surface, Centre de recherché du CHUQ, H?pital Saint‐Fran?ois d'Assise, 10 rue de l'Espinay, Québec, Canada G1L 3L5;5. INSERM U1026, Bordeaux F‐33076, France 146 rue Leo Saignat, 33076 Bordeaux, Cedex, France, and Université Bordeaux, Bordeaux F‐33076, France 146 rue Leo Saignat, 33076 Bordeaux, Cedex, France, and CNRS UMR 5248 CBMN, Institut Européen de Chimie et Biologie (IECB), Université de Bordeaux 1, 2 rue Robert Escarpit, F‐33607 Pessac, Cedex, France |
| Abstract: | HSVEC behavior under physiological shear stress in vitro is investigated on PET surfaces micropatterned with both RGDS and WQPPRARI peptides. This technique allows (i) creating geometries on surface to guide cell orientation under shear stress and (ii) controlling surface chemical composition in order to modulate cell behavior. Under shear stress, endothelial cells adhere on patterned PET surfaces and present a more rapid orientation in flow direction in comparison to cells cultured on homogeneous surfaces. Micropatterned surfaces presenting a large surface area ratio of RGDS/WQPPRARI peptides induce fibrillar adhesion, while surfaces presenting an equal RGDS/WQPPRARI peptides surface area ratio preferentially induce focal adhesion.  |
| Keywords: | adhesion biomaterials microstructure peptides shear |