Detection and quantitation of a pheomelanin component in melanin pigments using pyrolysis–gas chromatography/tandem mass spectrometry system with multiple reaction monitoring mode |
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Authors: | Anna Dzierżęga‐Lęcznar Slawomir Kurkiewicz Krystyna Stępień |
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Affiliation: | Department of Instrumental Analysis, Faculty of Pharmacy, Medical University of Silesia, , Katowice, Poland |
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Abstract: | Here, we describe the reliable method for the detection and quantitation of a pheomelanin component in melanin pigments. Synthetic melanins with various contents of pheomelanin‐type structural units were thermally degraded, and the multiple reaction monitoring mode was applied to detect the pheomelanin markers in the pyrolysates by GC/MS/MS. The method allowed the specific detection and quantitation of a pheomelanin component in melanin with the incorporation of pheomelanin‐type units as low as 0.05%. Considering highly universal character of the pheomelanin markers, the method could be applied for structural studies of natural melanin pigments being mixtures of eumelanin and pheomelanin. Copyright © 2012 John Wiley & Sons, Ltd. |
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Keywords: | melanin pheomelanin GC/MS/MS triple quadrupole multiple reaction monitoring mode |
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