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Chemotherapy control by breath profile with application of SPME‐GC/MS method |
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| Authors: | Agnieszka Ulanowska Ewa Trawińska Piotr Sawrycki Bogusław Buszewski |
| Affiliation: | 1. Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, , Torun, Poland;2. Department of Lung Disease, Collegium Medicum, Nicolaus Copernicus University, , Torun, Poland;3. Department of Cancer Chemotherapy, Collegium Medicum, Nicolaus Copernicus University, , Torun, Poland |
| Abstract: | Chemotherapy used as a treatment against lung cancer has influence on metabolic processes occurring in healthy cells. The changes of biochemical pathways proceeded inside cells might be observed in expired air. In the experiment, breath analysis was carried out before and after anticancer therapy. Expired air samples were collected from 22 patients with a biopsy confirmed lung cancer. Volatile organic compounds present in breath were analyzed by gas chromatography/mass spectrometry. For enrichment of analytes solid‐phase microextraction technique was applied. Eight fibers covered by different sorbents were tested. Carboxen‐polydimethylsiloxane fiber revealed the highest extraction efficiency in relation to analytes in breath. The data showed that cytostatic drugs increase the concentration of acetone and isoprene in the breath collected after chemotherapy. Volatile metabolites of administrated drugs were not identified in expired air. |
| Keywords: | Breath analysis Chemotherapy Lung cancer Solid‐phase microextraction (SPME) Volatile organic compounds (VOCs) |