9,10‐Diphenylanthracene as a matrix for MALDI‐MS electron transfer secondary reactions |
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Authors: | M Nazim Boutaghou Richard B Cole |
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Institution: | 1. Department of Chemistry, University of New Orleans, , New Orleans, LA, 70148;2. Laboratoire de Chimie Structurale Organique et Biologique, Institut Parisien de Chimie Moléculaire, Université Pierre et Marie Curie‐Paris 6, , 75252 Paris, France |
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Abstract: | The most common secondary‐ionization mechanism in positive ion matrix‐assisted laser desorption/ionization (MALDI) involves a proton transfer reaction to ionize the analyte. Peptides and proteins are molecules that have basic (and acidic) sites that make them susceptible to proton transfer. However, non‐polar, aprotic compounds that lack basic sites are more difficult to protonate, and creating charged forms of this type of analyte can pose a problem when conventional MALDI matrices are employed. In this case, forming a radical molecular ion through electron transfer is a viable alternative, and certain matrices may facilitate the process. In this work, we investigate the performance of a newly developed electron‐transfer secondary reaction matrix: 9,10‐diphenylanthracene (9,10‐DPA). The use of 9,10‐DPA as matrix for MALDI analysis has been tested using several model compounds. It appears to promote ionization through electron transfer in a highly efficient manner as compared to other potential matrices. Thermodynamic aspects of the observed electron transfers in secondary‐ionization reactions were also considered, as was the possibility for kinetically controlled/endothermic, electron‐transfer reactions in the MALDI plume. Copyright © 2012 John Wiley & Sons, Ltd. |
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Keywords: | MALDI electron transfer matrix secondary reaction 9 10‐diphenylanthracene endothermic |
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