1. BioTime Inc., 1301 Harbor Bay Parkway, Alameda, CA 94502, USA;2. ThermoFisher Scientific Inc., 925 West 1800 South, Logan, UT 84321, USA;3. Primogenix Inc., 85 Clear Star Dr., Gravios Mills, MO 65037, USA;4. Center for Biomaterials, Department of Reconstructive Sciences, University of Connecticut Health Center, Farmington, CT 06030, USA
Abstract:
These studies provide evidence for the ability of a commercially available, defined, hyaluronan‐gelatin hydrogel, HyStem‐C?, to maintain both mouse embryonic stem cells (mESCs) and human induced pluripotent stem cells (hiPSCs) in culture while retaining their growth and pluripotent characteristics. Growth curve and doubling time analysis show that mESCs and hiPSCs grow at similar rates on HyStem‐C? hydrogels and mouse embryonic fibroblasts and Matrigel?, respectively. Immunocytochemistry, flow cytometry, gene expression and karyotyping reveal that both human and murine pluripotent cells retain a high level of pluripotency on the hydrogels after multiple passages. The addition of fibronectin to HyStem‐C? enabled the attachment of hiPSCs in a xeno‐free, fully defined medium.
