1. Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8‐1 Kawadacho, Shinjuku, Tokyo 162‐8666, Japan;2. Graduate School of Pharmaceutical Sciences, Keio University, 1‐5‐30 Shibakoen, Minato, Tokyo 105‐8512, Japan
Abstract:
Thermoresponsive surfaces are prepared via a spin‐coating method with a block copolymer consisting of poly(N‐isopropylacrylamide) (PIPAAm) and poly(butyl methacrylate) (PBMA) on polystyrene surfaces. The PBMA block suppresses the removal of deposited PIPAAm‐based polymers from the surface. The polymer coating affects the temperature‐dependent cellular behavior of the surfaces with respect to protein adsorption. By adjusting layer thicknesses, PBMA‐b‐PIPAAm‐coated surfaces are optimized to regulate the adhesion/detachment of cells by temperature changes. Thus, thermoresponsive polymer‐coated surfaces are able to harvest contiguous cell sheets with their basal extracellular matrix proteins.
