Hyperbranched Polylysine: A Versatile,Biodegradable Transfection Agent for the Production of Recombinant Proteins by Transient Gene Expression and the Transfection of Primary Cells
1. Laboratoire des Polymères, Institut des Matériaux et Institut des Sciences et Ingénierie Chimiques, école Polytechnique Fédérale de Lausanne (EPFL), Batiment MXD, Station 12, CH‐1015 Lausanne, Switzerland;2. Laboratory of Cellular Biotechnology, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, CH‐1015 Lausanne, Switzerland
Abstract:
The feasibility of a new transfection agent, HBPL, for the production of recombinant IgG antibody via TGE as well as for the transfection of primary cells is studied. Under the conditions investigated, transfection of CHO‐DG44 cells using HBPL results in IgG yields that are comparable to those obtained with PEI. In experiments with CHO‐K1 cells and MEFs the use of HPBL allows to achieve transfection efficiencies comparable to or better than those obtained with PEI or Fugene®. HBPL‐mediated transfection does not require complex pre‐formation, works well in serum‐containing media and is biodegradable, which may prevent cumulative cytotoxicity and facilitates downstream processing.
