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Determination of ginkgolide A,B, and bilobalide in<Emphasis Type="Italic"> biloba</Emphasis> L. extracts by microdialysis-HPLC
Authors:Houn-Lin?Chiu  Hui-Ya?Lin  Email author" target="_blank">Thomas?Ching-Cherng?YangEmail author
Institution:(1) Department of Chemistry, National Kaohsiung Normal University, 116 Ho-Ping 1st Rd., Kaohsiung, Taiwan
Abstract:A method has been developed for measuring ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts by using a self-assembled microdialysis device coupled on-line to high-performance liquid chromatography. The optimal conditions for dialysis efficiency and chromatographic analysis were investigated. The results show that the dialysis efficiencies for ginkgolide A, B, and bilobalide are 97.8–100.4%. For the spike concentration of 45 mg L–1, the recoveries of ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts were at 97–100.7% with 3.1–4.7% RSD. The proposed method has therefore been shown to be applicable to the analysis of ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts.
Keywords:High-performance liquid chromatography  Microdialysis  Ginkgolide A  Ginkgolide B  Bilobalide
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